SPEAKER: Dr. Bruno Moretti (UC Berkeley / Chan Zuckerberg Biohub)

TITLE: “Single-cell transcriptional dynamics in living zebrafish embryos

DATE: Monday, June 3, 12 PM.

VENUE:  IBioBA´s seminar room. Godoy Cruz 2390, C1425FQD, Buenos Aires, Argentina.

Abstract: The ability to quantify transcriptional dynamics in individual cells via live imaging has revolutionized our understanding of gene regulation. However, such measurements are lacking in the context of vertebrate embryos. We addressed this deficit by applying MS2-MCP mRNA labeling to the quantification of transcription in zebrafish. We developed a platform of transgenic organisms, light sheet fluorescence microscopy, and optimized image analysis that enables visualization and quantification of MS2 reporters. With these tools, we obtained the first single-cell, real-time measurements of transcriptional dynamics of the segmentation clock. Our measurements challenge the traditional view of smooth clock oscillations and instead suggest a model of discrete transcriptional bursts that are organized in space and time. Together, these results highlight how measuring single-cell transcriptional activity can reveal unexpected features of gene regulation and how this data can fuel the dialogue between theory and experiment.