Lucía Canedo, Melina D. Muñoz Bernart, Nicolás Budnik.
We study molecular and cellular mechanisms involved during the dynamic regulation of cell plasticity and how the tumor microenvironment regulates the expression of key genes in this process. Cell plasticity in tumors contributes to the heterogeneous cell population observed in tumors, and consequently, their recurrence after treatment and dissemination.
Cancer Stem Cells (CSC) are those tumor cells responsible for cell plasticity and express core pluripotency factors and epigenetic regulators leading to the acquisition/maintenance of a malignant cell phenotype.
Also, TGF-beta family of proteins, as part of the tumor microenvironment and active in tumors, contribute to the maintenance of the stem-like properties.
Our research includes the use of human tumor samples and mouse xenograft models, and three-dimensional (3D) cultures of cancer cells (enrichment of CSC) that simulate in vitro the conditions of cell plasticity in the tumor. To conceive and understand the organization and dynamic progression of tumor cells in these models, we carried out a multidisciplinary approach and advanced microscopy methods.
Publications related to this project
- Ferreyra Solari NE, Belforte FS, Canedo L, Videla-Richardson GA, Espinosa JM, Rossi M, Serna E, Riudavets MA, Martinetto H, Sevlever G and Perez-Castro C.
The NSL Chromatin-Modifying Complex Subunit KANSL2 Regulates Cancer Stem–like Properties in Glioblastoma That Contribute to Tumorigenesis.
Cancer Research 76: 5383-5394 (2016)
- Rohr, C.O.; Parra, R.G.;Yankilevich, P.;Perez Castro, C.
INSECT: In silico search for co-occurring transcription factors.
Bioinformatics 29:2852-2858 (2013)
- Perez Castro, C.; Renner, U.; Haedo, M.; Gunter, K.; Stalla, G.K.; Arzt, E.
Cellular and Molecular Specificity of Pituitary Gland Physiology.
Physiological Reviews 92:1-38 (2012)
- Perez-Castro, C., Piscopo, D, Nakagawa, T, Derynck, R.
Cornichon regulates transport and secretion of TGF-alpha related proteins in metazoan cells.
Journal of Cell Science 120:2454-2466 (2007)
- Graciarena, M, Carbia-Nagashima, A, Onofri, C, Perez-Castro, C, Giacomini, D, Renner, U, Stalla, GK, Arzt, E.
Involvement of the gp130 cytokine transducer in MtT/S pituitary somatotroph tumor development in an autocrine-paracrine model.
European Journal of Endocrinology 151:595-604 (2004)
- Nagashima, AC; Giacomini, D; Perez-Castro, C; Páez-Pereda, M; Renner, U; Stalla, GK, Arzt, E.
Transcriptional regulation of IL-6 in pituitary folliculo-stellate TtT/GF cells.
Mol Cell Endocrinology 201:47-56 (2003)